The first genome of a living organism sequenced in 1995 was:

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The first genome of a living organism sequenced in 1995 was the bacterium Haemophilus influenza. Haemophilus influenza is a gram-negative, facultatively anaerobic, coccobacillus bacteria. The bacterium is the first free-living organism, whose genome was sequenced. Before the sequencing of the H. influenza genome, Frederick Sanger and his team sequenced the full genome of virus phiX174.

The genome of H. influenza was sequenced at The Institute for Genomic Research, Maryland by American biotechnologist Craig Venter and his team. Now the institute is a part of J Craig Venter Institute. The strain of H. influenza bacteria used for the sequencing was Rd KW20. The DNA library for sequencing was provided by American microbiologist and Nobel laureate Hamilton Othanel Smith.

The genome of bacterium H. influenza consists of 1,830,138 bp of DNA. The genome contains 1604 protein-coding genes, 57 tRNA genes, 117 pseudogenes, and 23 other RNA genes. The sequencing was performed through the whole-genome shotgun. The study was published in Science magazine (1995). The Genome Sequence DataBase accession number for the whole genome sequence of H influenza is L42023>.