The plasmid pBR322 has antibiotic resistance genes for:

The plasmid pBR322 is a prokaryotic cloning vector. The pBR322 plasmid named as the p stands for “plasmid,” and the BR stands for the creator of the plasmid “Bolivar” and “Rodriguez” and the 322 stands for the transformed colonies used for sequencing. The length of plasmid pBR322 is 4361 base pairs.

The plasmid pBR322 has two antibiotic resistance genes. They are the bla gene and the tetA gene. The protein encoded by the bla gene is ampicillin resistance (Amp^R) which provides resistance against antibiotic ampicillin. The protein encoded by the tetA gene is tetracycline resistance (Tet^R) which provides resistance against antibiotic tetracycline.

Both the antibiotic resistance genes act as selectable markers. Further, these two genes contain unique restriction sites for many restriction enzymes.