Mol Bio

During amplification of gene using PCR, Taq polymerase is used between

spadmanabh009 — Wed, 13 Apr 2022 03:05:32 +0000

[HDquiz quiz = "1967"] Polymerase chain reaction or PCR is a technique to amplify a specific segment of DNA using a pair of primers and Taq DNA polymerase enzyme. The binding of primers on the DNA segment is called the Annealing. After annealing of primers, the Taq DNA polymerase binds and extends the DNA fragments. …

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The genome of which of the following multicellular organism was first sequenced?

spadmanabh009 — Thu, 07 Apr 2022 07:10:42 +0000

[HDquiz quiz = "1954"] The genome of the first multicellular organism sequenced was Caenorhabditis elegans or C elegans. The genome of C elegans was sequenced in the year 1998. The size of the genome is about 97 Mb. The worm contains six pairs of chromosomes. The genome codes for 20,470 protein-coding genes.

The first codon discovered by Nirenberg and Matthaei was :

spadmanabh009 — Wed, 23 Feb 2022 15:31:01 +0000

The Nirenberg and Matthaei experiment was performed by American biologist Marshall W. Nirenberg (1927-2010) and German biologist J. Heinrich Matthaei in 1961. Matthaeiat was a postdoc fellow in the lab of Nirenberg at the National Institutes of Health, USA. The Nirenberg and Matthaei experiment was a famous experiment used to decipher the genetic code. In this experiment, Nirenberg and Matthaei used bacterial …

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The genome of E coli contains 4 million base pairs or 4×10^6 bp. The number of turns of DNA present is

spadmanabh009 — Wed, 23 Feb 2022 02:32:21 +0000

The genome of E coli contains 4 million base pairs or 4×106 bp. The number of turns of DNA present is 400,000 turns or (4×105) turns. In general, in the B form of DNA, one turn of DNA contains 10 bp. Therefore, the number of turns present in the genome is around 10 times less …

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The histone protein having the lowest molecular weight is

spadmanabh009 — Fri, 18 Feb 2022 11:04:54 +0000

Histones are basic proteins that in association with DNA form a structure known as the nucleosome. Nucleosomes help in the condensation of DNA into chromatin inside the eukaryotic nucleus. The nucleosome consists of four types of histone proteins. They are H2A, H2B, H3, and H4. A total of eight histones proteins are present i.e. 2 …

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Which of the following tool is not used to analyze the restriction enzymes sites of a DNA sequence?

spadmanabh009 — Mon, 14 Feb 2022 13:42:26 +0000

The bioinformatics tool that is not used to analyze the restriction enzymes sites of a DNA sequence is EuGene. NEBcutter, Webcutter, and WatCut are the bioinformatics online tools used for analyzing and predicting the cutting site by restriction endonucleases. On the other hand, the bioinformatics online tool EuGene is used for the prediction of genes …

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An analysis of a DNA (double-stranded) sample yielded 18% cytosine. What would be the percentage of other bases on this sample?

spadmanabh009 — Sat, 05 Feb 2022 06:49:07 +0000

Erwin Chargaff proposed a rule called the Chargaff’s rules which states that in a double-stranded DNA segment, the ratio of purine bases (Adenine+Guanine) and the pyrimidine bases (Cytosine+Thymine) is equal to 1.0 and the number of Adenine is equal to the Thymine and the number of Guanine is equal to the number of Cytosine. In the …

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Genetically active area of chromosomeis called:

spadmanabh009 — Fri, 04 Feb 2022 03:49:57 +0000

DNA is tightly packed in the form of condensed chromatin structure to form chromosomes. The nucleosome is the unit of chromatin that form from eight core histone (2 units of each H2A, H2B, H3, and H4) and 147 bp of DNA wrapped around it. Some of the areas of chromatin are lightly packed. This lightly …

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is called:"

7-methyl guanosine (m7G) capping protects the mRNA from

spadmanabh009 — Sun, 30 Jan 2022 07:36:18 +0000

After transcription, a cap forms at the 5′ end of the mRNA in eukaryotes. The cap forms by the attachment of 7-methyl guanosine (m7G). 7-methyl guanosine is a modified guanosine nucleotide where a methyl (-CH3) group is attached at the 7′ position of guanosine nucleotide. During the capping, the 5′ end of the 7-methyl guanosine …

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What is the pitch of B-DNA?

spadmanabh009 — Fri, 28 Jan 2022 13:28:50 +0000

B-DNA is the right-handed DNA helix that is the original model of DNA proposed by JD Watson and F Crick. In the B-DNA model, two DNA strands twist around each other spirally in a right-handed way with 10 base pairs per turn. The distance between the two base pairs (rise) is 0.34 nm. The pitch …

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