Which of the following tool is not used to analyze the restriction enzymes sites of a DNA sequence?

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The bioinformatics tool that is not used to analyze the restriction enzymes sites of a DNA sequence is EuGene. NEBcutter, Webcutter, and WatCut are the bioinformatics online tools used for analyzing and predicting the cutting site by restriction endonucleases.

On the other hand, the bioinformatics online tool EuGene is used for the prediction of genes in eukaryotic organisms.