The Cre-Lox system uses Cre recombinase.
The Cre-Lox system uses Cre recombinase. The Cre-Lox system consists of a single enzyme, Cre recombinase, a DNA recombinase that recombines two short target DNA sequences called the Lox sequences, a short DNA sequence derived from bacteriophage P1. The Cre protein is encoded by the gene or locus originally named as “Causes recombination”. The Cre protein consists of 4 subunits with 343 amino acids (38kDa). The protein contain two domains: The larger carboxyl terminal domain, and smaller amino terminal domain. The C domain contain the catalytic site of the enzyme that catalyses the site specific recombination of two DNA recognition sites having the LoxP sites.
Cre-Lox recombination is a site-specific recombinase technology used in the field of biotechnology to carry out insertions, deletions, inversions, and translocations at specific target sites in the DNA of cells.
The Cre-Lox technology was patented by DuPont Pharmaceuticals.
